|
KMID : 0043319940170060452
|
|
Archives of Pharmacal Research
1994 Volume.17 No. 6 p.452 ~ p.457
|
|
|
Ricin A Immunotoxins of IgG and Fab of Anti-CALLA Monoclonal Antibody: Effect of Water Soluble Long-chain SPDP on Conjugate Yield, Immunoselectivity and Cytotoxicity
|
|
Woo Byung-Ho
Lee Jung-Tae
Lee Kang-Choon
|
|
|
Abstract
|
|
|
|
The water soluble long-chain crosslinker, sulfo-succinimidyl-6-[3’-(2-pyridyldithio)-propion-amido]hexanoate (S-LC-SPDP) was used to prepare ricin A chain (RAT) immunotoxins constructed with whole igG and Fab fragments of the anti-common acute lymphoblastic leukemiz antigen (CALLA)monoclonal antibody. In this study, a) S-LC-SPDP modification efficiencies immunoreactivity and cytotoxicity of immunotoxins constructed were examined. IgG-RTA and Fab-RTA immunotoxins were prepared with 67.3% and 57.0% conjugation yields, respectively. These long spacer intemolecular linked immunotoxins were selectively immunoreactive and to antigen K562 cells. Both IgG-RAT and Fab-RAT immunotoxins were 210-and 45-fold more active than intavt RAT in vitro, respectively.
|
|
|
KEYWORD
|
|
|
lmmuontoxin, Rich A chain, CALLA, Monoclonal antibody, Fab fragment, Crosslinking, Sulfo-LC-SPDP, Drug targeting
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|